MBD Enrichment - Ethanol Precipitation of C.virginica MBD samples

After completing two rounds of MBD enrichment (first 12 samples on 20190307 and the second 12 samples on 20190311), I performed ethanol precipitation, per the MethylMiner instructions with the following modifications:

• after additions of glycogen, sodium acetate and ethanol, incubated samples O/N at -80^oC

• after incubation, the two elutions were consolidated in the following fashion:

  • the first of each of the two elutions was pelleted, supe discarded, and second elution transferred to the pellet
  • second elution was pelleted in same tube as first

• spins were performed at 13,000g

Samples were resuspended in 25uL of PCR H₂O.

Currently, don’t have appropriate tubes for quantification, so will do so once they arrive.

Samples were stored in the small -20^oC freezer in the following box (Google Sheet):

• Sam gDNA Box 3