RNA Isolation and Quantification - C.bairdi Hemocyte Pellets in RNAlater

Isolated RNA from the following hemolymph pellet samples:

• 6174_233_12

• 6258_261_12

• 6270_269_12

• 6161_302_12

• 6115_307_12

• 6206_319_12

• 6241_323_12

• 6158_324_12

• 6272_338_12

• 6152_344_12

• 6275_372_12

• 6218_373_12

• 6153_404_26

• 6132_406_26

• 6162_407_26

• 6178_410_26

• 6163_424_26

• 6140_429_26

• 6174_433_26

• 6199_445_26

• 6156_446_26

• 6106_450_26

• 6151_454_26

• 6177_456_26

• 6119_461_26

• 6124_464_26

• 6134_465_26

• 6168_466_26

• 6152_467_26

• 6121_472_26

• 6205_475_26

• 6155_476_26

• 6143_479_26

• 6158_483_26

• 6169_486_26

Isolated RNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.

• Used 35uL from each RNAlater/hemocyte slurry.

• Mixed with equal volume of H₂O (35uL).

• Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.

• Performed on-column DNase step.

• RNA was eluted in 15uL H₂O

RNA was quantified on the Roberts Lab Qubit 3.0 using the RNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.

RNA was stored in the -80_{oC freezer} in Shellfish RNA Box #7 and Shellfish Box #8.

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RESULTS

Qubit restuls (Google Sheet):

• 20200124_qubit_cbai_RNA

Sample ID	RNA
6258_261_12	10.6
6270_269_12	31.7
6161_302_12	8.04
6115_307_12	7.89
6206_319_12	11.2
6241_323_12	22.2
6158_324_12	16.5
6272_338_12	10.6
6152_344_12	6.65
6275_372_12	10.1
6218_373_12	14.3
6174_233_12	17.8
6153_404_26	12.2
6132_406_26	12.3
6162_407_26	14.5
6178_410_26	Out of range
6163_424_26	21.4
6140_429_26	12
6174_433_26	17.6
6199_445_26	20.4
6156_446_26	20.7
6106_450_26	25.4
6151_454_26	11.4
6177_456_26	19.9
6119_461_26	11.2
6124_464_26	19.1
6134_465_26	9.1
6168_466_26	18.4
6152_467_26	5.45
6121_472_26	8.57
6205_475_26	13.1
6155_476_26	15.3
6143_479_26	15.3
6158_483_26	13.3
6169_486_26	5.87