Isolated RNA from the following hemolymph pellet samples:
6202_337_12
6142_474_26
6144_477_26
6122_489_26
6176_490_26
6136_491_26
6137_500_26
6125_503_26
6145_505_26
6131_507_26
6159_508_26
6206_509_26
6150_511_26
6161_512_26
6115_514_26
6179_515_26
6172_517_26
Isolated RNA using the Quick DNA/RNA Microprep Kit (ZymoResearch; PDF) according to the manufacturer’s protocol for liquids/cells in RNAlater.
Used 35uL from each RNAlater/hemocyte slurry.
Mixed with equal volume of H2O (35uL).
Retained DNA on the Zymo-Spin IC-XM columns for isolation after RNA isolation.
Performed on-column DNase step.
RNA was eluted in 15uL H2O
RNA was quantified on the Roberts Lab Qubit 3.0 using the RNA High Sensitivity Assay (Invitrogen), using 2uL of each sample.
RNA was stored in the -80oC freezer in Shellfish Box #8.
RESULTS
Qubit restuls (Google Sheet):
| Sample ID | RNA |
|---|---|
| 6202_337_12 | 23.7 |
| 6142_474_26 | 14.4 |
| 6144_477_26 | 20.3 |
| 6122_489_26 | 8.32 |
| 6176_490_26 | 10.7 |
| 6136_491_26 | 7.37 |
| 6137_500_26 | 13.8 |
| 6125_503_26 | 7.12 |
| 6145_505_26 | 4.7 |
| 6131_507_26 | 8.9 |
| 6159_508_26 | 7.74 |
| 6206_509_26 | 9.75 |
| 6150_511_26 | 4.12 |
| 6161_512_26 | 10.9 |
| 6115_514_26 | 5.96 |
| 6179_515_26 | 7.23 |
| 6172_517_26 | Out of range |