Today we received the whole genome bisulfite sequencing (WGBS) from the 24 C.gigas diploid-triploid samples subjected to different pH that were submitted 20200824. The lengthy turnaround time was due to a bad lot of reagents, which forced them Zymo to find a different manufacturer in order to generate libraries.
Sequencing consisted of WGBS 150bp paired end (PE) reads for each library. All files were downloaded to the C_gigas folder on Owl(Synology server). MD5 checksums were confirmed:
Principal spreadsheet for this project was updated (Google Sheet):
Have added files to our high-throughput sequencing database (Google Sheet):
Next up:
Submit to NCBI sequence read archive (SRA).
| Zymo_ID | Sample_ID | Ploidy | pH_treatment |
|---|---|---|---|
| zr3644_1 | 2N_HI_5 | diploid | high |
| zr3644_2 | 2N_HI_8 | diploid | high |
| zr3644_3 | 2N_HI_9 | diploid | high |
| zr3644_4 | 2N_HI_10 | diploid | high |
| zr3644_5 | 2N_HI_11 | diploid | high |
| zr3644_6 | 2N_HI_12 | diploid | high |
| zr3644_7 | 2N_LOW_1 | diploid | low |
| zr3644_8 | 2N_LOW_2 | diploid | low |
| zr3644_9 | 2N_LOW_3 | diploid | low |
| zr3644_10 | 2N_LOW_4 | diploid | low |
| zr3644_11 | 2N_LOW_5 | diploid | low |
| zr3644_12 | 2N_LOW_6 | diploid | low |
| zr3644_13 | 3N_HI_2 | triploid | high |
| zr3644_14 | 3N_HI_3 | triploid | high |
| zr3644_15 | 3N_HI_5 | triploid | high |
| zr3644_16 | 3N_HI_8 | triploid | high |
| zr3644_17 | 3N_HI_10 | triploid | high |
| zr3644_18 | 3N_HI_11 | triploid | high |
| zr3644_19 | 3N_LOW_6 | triploid | low |
| zr3644_20 | 3N_LOW_7 | triploid | low |
| zr3644_21 | 3N_LOW_8 | triploid | low |
| zr3644_22 | 3N_LOW_10 | triploid | low |
| zr3644_23 | 3N_LOW_11 | triploid | low |
| zr3644_24 | 3N_LOW_12 | triploid | low |