Submitted the M.magister MBD-BSseq libraries created 20201124 using the 4nM aliquots created for the MiSeq test run on 20201202 to the Univ. of Oregon GC3F sequencing core.
For library pooling, I used an updated set of calculations determined by Mac to help equalize individual library contributions, based on the results from the MiSeq run:
| Sample | Volume_4nMsample_toBalance_uL | |
|---|---|---|
| CH01-06 | 2.43 | |
| CH01-14 | 3.1 | |
| CH01-22 | 2.58 | |
| CH01-38 | 1.63 | |
| CH03-04 | 1.66 | |
| CH03-15 | 4.03 | |
| CH03-33 | 1.71 | |
| CH05-01 | 2.1 | |
| CH05-06 | 4.38 | |
| CH05-21 | 1.84 | |
| CH05-24 | 2.98 | |
| CH07-06 | 1.79 | |
| CH07-11 | 2.83 | |
| CH07-24 | 2.98 | |
| CH09-02 | 1.36 | |
| CH09-13 | 4.57 | |
| CH09-28 | 1.4 | |
| CH10-01 | 1.29 | |
| CH10-08 | 1.83 | |
| CH10-11 | 0.99 |
Full Google Sheet for this project: