Reverse Transcription - C.gigas RNA from Noah’s Heat-Mechanical Stress Project

After isolating ctenidia RNA on 20230712 and then quantifying the RNA earlier today, I needed to perform reverse transcription to have cDNA for subsequent qPCRs. Reverse transcription was performed using oligo dT primers using M-MLV RT (Promega), per the manufacturer’s recommendations. Used 100ng of RNA in each reaction. All reactions were done on ice in 0.5uL PCR tubes.

• 20230713-cgig-ctenidia-Noah-cDNA-calcs (Google Sheet)

In preparation for qPCR and primer testing, 2uL from each sample was pooled together in a separate 0.5uL tube. All cDNA was stored in Noah’s -80^oC project box.