Steven asked me to generate cDNA (GitHub Issue) from Crassostrea gigas (Pacific oyster) RNA, as part of Matt George’s polyIC project. Before starting reverse transcription, I felt it would be best to quantify the RNA using the Qubit 3.0 using the Broad Range Assay. Steven and I discussed which samples to use earlier this week.
Used 1uL of each sample.
See Results section below for sample/tube labels.
RESULTS
- 20230721-qubit-cgig-polyIC-RNA (Google Sheet)
All samples had ample amount of RNA. Will proceed with reverse transcription.