Bisulfite Conversion - Lake Trout gDNA Using EZ DNA Methylation Lightning Kit

2024
lake trout
Salvelinus namaycush
bisulfite
EZ DNA Methylation Lightning Kit
Author

Sam White

Published

August 13, 2024

INTRO

After isolating gDNA on 20240712 (Notebook), the next step was to perform bisulfite conversion.

METHODS

I used 500ng of gDNA from each sample for bisulfite conversion using the EZ DNA Methylation Lightning Kit (ZymoResearch), according to the manufacturer’s protocol. Samples were eluted with 10uL of the M-Elution Buffer and stored @ -20oC in the same box as the source DNA.

Calculations for input DNA volumes are here:

20240813-snam-bs.dna-calcs (Google Sheet)

DICUSSION

Next step will be to perform qPCR to amplify the C1q gene using the primers designed on 20240729 (Notebook), for eventual sequencing.