INTRO

I had previously isolate Salvelinus namaycush (Wikipedia) liver gDNA on . Steven asked me to concentrate the gDNA (GitHub Issue) to ~800 - 1000ng/uL, per a request of the sequencing facility.

MATERIALS & METHODS

All samples were concentrated in a SpeedVac, under vacuum, at the medium heat setting. The samples were monitored periodically to ensure they did not dry out completely. Once the sample volumes were reduced to ~10uL (this would concentrate all the samples ~4X which should achieve the target concentrations ). The process took ~5hrs. The samples were removed and stored at -20°C until further use.