RNA Isolation - C.gigas Lifestage Carryover Samples Using Direct-zol RNA MiniPrep Plus Kit

2025
Crassostrea gigas
Pacific oyster
RNA isolation
Direct-zol RNA MiniPrep Plus Kit
Magallana gigas
Author

Sam White

Published

May 13, 2025

INTRO

I was asked to isolate RNA from a list of 60 samples (GitHub Issue) from a number of Crassostrea gigas (Pacific oyster) spat and seed that had been collected as part of the project-gigas-carryover (GitHub repo). Yesterday, I homogenized samples in TriZol and stored them at -20oC. RNA from the other samples listed in this project were isolated yesterday immediately after homogenization (Notebook entry).

Here’s the list of samples I isolated RNA from today:

78 79 81 83 86 88 89 92 93 96 97 98

RNA Isolation

RNA isolations were conducted using the Direct-zol RNA MiniPrep Plus Kit (Zymo Research) according to the manufacturer’s protocol, including the optional DNase treatment step. All samples were eluted with 50 µL of nuclease-free water.

Sample Storage

Samples were stored on ice in preparation for quantification (Notebook entry).