INTRO
Steven asked me to perform resazurin assays on “selected” juvenile Ruditapes philippinarum (Manila clam) exposed to 36oC acute heat stress. The goal of the experiment is to compare metabolic activity between the “selected” clams and control clams when exposed to heat stress. This is similar to the previous experiment at 36oC Day 0 Notebook Entry and Day 1 Notebook Entry, but with a set of larger clams.
MATERIALS & METHODS
Clams were processed in 12-well plates and submerged in 5.0mL of resazurin working solution stored at 4oC,prepared on 20260330 (notebook entry).
Plates were measured every 30mins on a Synergy HTX (Agilent) plate reader.
Plate layout was randomized; Steven is currently the only person who knows the treatment assignments.
The following wells lost volume - possibly due to clam spitting?
- GA3
- JB1
- KA2
- LA4
Plate Temps
Wells were spot checked and range noted in table below.
| PLATE | TIME | TEMP |
|---|---|---|
| G | 0 | 11-12 |
| G | 0.5 | 14-16 |
| G | 1 | 24-30 |
| G | 1.5 | 27-30 |
| G | 2 | 31-32 |
| G | 2.5 | 31-32 |
| G | 3 | 31-32 |
| G | 3.5 | 31-32 |
| G | 4 | 33 |
RESULTS
Data are available here:
At the time of this writing, there is no plate layout file available for this experiment, so it’s not possible to determine which wells correspond to which clams. Steven is working on creating a plate layout file, which will be added to the GitHub repository when it’s available.