INTRO

In preparation for testing out some new Manila clam primers (GitHub Issue), Steven asked that I isolate some RNA from some Manila clams we currently have in the tank room (GitHub Issue). However, I remembered we already had some old Manila clam RNA from waaaay back in 2008. The RNA is gill RNA from an experiment where some clams (labeled with S) were spun for 1min in the salad spinner and corresponding controls.

Due to the age, and the fact the RNA had previously been quantified using the NanoDrop (which is not particularly accurate), I opted to quantify the RNA using the Qubit High Sensitivity (HS) RNA Assay.

MATERIALS & METHODS

Samples were quantified using the hsRNA Qubit Assay with the Roberts’ Lab Qubit 3.

Used 1uL of each sample.

RNA was returned to the -80^oC in Shellfish RNA Box #3 (Google Sheet)

RESULTS

Qubit data:

20261027-rphi-qubit-RNA-hs-gill (Google Sheet)

Sample_ID	Original sample conc. (ng/uL)
RNA Manila clam gill C1	89.8
RNA Manila clam gill C2	184
RNA Manila clam gill C3	158
RNA Manila clam gill C4	184
RNA Manila clam gill C5	200
RNA Manila clam gill S1	70
RNA Manila clam gill S2	73.8
RNA Manila clam gill S3	88.2
RNA Manila clam gill S4	108
RNA Manila clam gill S5	130

I created a pool of RNA for subsequent use in making cDNA using 1uL of each sample. It was also stored in the same -80^oC Shellfish RNA Box #3 (Google Sheet)