Continued Illumina library prep of bisulfite-treated DNA samples (400ppm and 1000ppm; from 20150115) with Methylamp DNA Modification Kit (Epigentek) (PDF). Performed bead clean up immediately after End Repair.
PCR cycles: 14
No other changes were made to the manufacturer’s protocol.
Epigentek Barcode Indices assigned, per their recommendations for using two libraries for multiplexing:
400ppm - barcode #6 - GCCAAT
1000ppm - barcode #12 - CTTGTA
The two libraries were stored @ -20C and will be quantified tomorrow.