Library Quality Assessment - C.gigas OA larvae Illumina libraries

2015
Crassostrea gigas larvae OA (2011) bisulfite sequencing
bioanalyzer
BS-seq
Crassostrea gigas
EpiNext Post-Bisulfite DNA Library Preparation Kit
Genomics Core Facility
larvae
library prep
OA
ocean acidification
Pacific oyster
University of Oregon
Author

Sam White

Published

March 2, 2015

Ran the 400ppm library preps on a DNA1000 Assay Chip (Agilent) on the Agilent 2100 Bioanalyzer.

Results:

Data File (XAD): 2100_expert_DNA_1000_DE72902486_2015-03-02_09-18-02.xad

Electropherogram overlay of both samples:

Red = 400ppm

Blue = 1000ppm

(http://eagle.fish.washington.edu/Arabidopsis/Bioanalyzer%20Data/20150302_BioanalyzerGigasLarvaeOA400_1000ppm.jpg)

Both libraries look good; no adaptor contamination (peak would be present at ~125bp), good library sizes.

Pooled equal quantities of each library, based off the concentration values above, to prepare the sample for sequencing.

Component Volume (μL) Quantity (ng)
400ppm library 10 14.7
1000ppm library 1.09 14.7
Buffer EB 7.81 N/A
1% Tween20 2.1 N/A
Total 21 N/A

The pooled libraries will be submitted tomorrow to the Genomics Core Facility at the Univ. of Oregon for high-throughput sequencing (100bp, SE) on the HiSeq2500 (Illumina). Sample order #2212.