Daily Bits - March 2024

20240326

project-gigas-carryover (GitHub Repo)

• Organized repo.
• Added READMEs.
• Finished qPCR notebook entry.

20240325

In lab

• Ran qPCRs (Notebook) for project-gigas-carryover (GitHub Repo).

20240320

Miscellaneous

• Made notebook posts for lab work from yesterday:
  • Isolated RNA for gigas carryover project (Notebook)
  • Reverse transcribed RNA for gigas carryover project (Notebook)

20240319

In lab

• Isolated RNA for gigas carryover project (Notebook)

• Reverse transcribed RNA for gigas carryover project (Notebook)

20240318

Miscellaneous

• Caught up on a bunch of purchasing issues/tickets.

CEABIGR (GitHub repo)

• Submitted sequencing data to NCBI SRA. (Notebook)

20240315

Science Hour

• Chatted with Chris. Discussed approaches for writing manuscripts to try to overcome writer’s block and/or motivation.

20240314

Lab Meeting

• Discussed 2nd half of “Tending Sweetgrass” in “Braiding Sweetgrass”

Miscellaneous

• Finished notebook entry for 2nd resazuring trial (a lot of frustrations with ChatGPT on the computation side of things…).

CEABIGR (GitHub repo)

• Worked on manuscript.

20240312

In Lab

• Conducted 2nd resazurin trial (Notebook).

20240311

Miscellaneous

• Directed Azenta to post invoices to UW Workday system.

• Added initial resazurin trial README.md to the project-gigas-carryover repo.

CEABIGR (GitHub repo)

• Began transferring sequencing data to NCBI.

20240308

Coral E5 (GitHub repo)

• Reviewed manuscript with Steven.

Miscellnaeous

• Finished up more ProCard stuff.

20240307

In lab

• Ran initial resazurin test (Notebook) on some small Crassostrea gigas (Pacific oyster) oysters provided by Steven.

• Lab meeting. (Otter AI summary)

• Received chemicals for resazuring assay. Got those entered into MyChem and entered into Workday.

20240306

CEABIGR (GitHub repo)

• Lengthy meeting to work extensively on manuscript - primarily documenting where code/data came from and organizing repo.

20240305

• Dealt with a bunch of annoying ProCard reconciliation stuff.

20240304

Miscellaneous

• Read the rest of “Tending Sweetgrass” section in “Braiding Sweetgrass”.

• Dealt with certificate issues for Eagle and Gannet. Despite “renewing” them, the renewal “broke” wget (can no longer download without the use of --no-check-certificate flag). Remembered that I’d had this issue before and tracked down a notebook entry that dealt with this issue. Needed to install intermediate certificates and that resolved the problem!

20240301

Miscellaneous

• Renewed Eagle server SSL certificate.

• Renewed Gannet server SSL certificate.

• Science Hour

  • Steven out of town, no show by anyone else.

CEABIGR (GitHub repo)

• Updated README files.