Chemical SOPs
University of Washington Lab Safety Manual
Chemical Material Safety Data Sheets (MSDS) on MyChem website.
UW Hazardous Waste Online Collection Request
RNAzol RT
Purpose: RNAzol RT is used for separating RNA from cells.
Specific Hazards: RNAzol RT is corrosive and harmful if inhaled.
Personal Protective Equipment:
-
REQUIRED:
-
Gloves
-
Chemical fume hood
-
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
- Liquid Waste:
- Transfer liquid waste to labeled container designated for RNAzol waste.
- Solid Waste:
- Tubes/tip/gloves with residual RNAzol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Isopropanol (2-propanol)
Purpose: Used in the precipitation of nucleic acids.
Specific Hazards: Highly flammable - keep away from ignition sources.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
-
WHEN SPLASH POTENTIAL EXISTS
-
Safety goggles
-
Lab Coat
-
Waste Disposal:
-
Liquid Waste:
- Transfer liquid waste to labeled container designated for isopropanol waste.
-
Solid Waste:
- Tubes/tip/gloves with residual isopropanol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Diethylpyrocarbonate (DEPC)
Purpose: Used as an RNase inhibitor during handling and storage of RNA.
Specific Hazards:
- Skin irritant and harmful if inhaled.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
- Chemical fume hood
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
- Liquid Waste:
- Transfer liquid waste to labeled container designated for DEPC waste.
- Solid Waste:
- Tubes/tip/gloves with residual DEPC should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
DNAzol
Purpose: DNAzol is used for separating DNA from cells.
Specific Hazards:
- DNAzol is corrosive.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
- Liquid Waste:
- Transfer liquid waste to labeled container designated for DNAzol waste.
- Solid Waste:
- Tubes/tip/gloves with residual DNAzol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Ethanol
Purpose: Used in the precipitation of nucleic acids.
Specific Hazards:
- Highly flammable - keep away from ignition sources.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
-
Liquid Waste:
- Transfer liquid waste to labeled container designated for ethanol waste.
-
Solid Waste:
- Tubes/tip/gloves with residual ethanol should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Phenol:chloroform:IAA (25:24:1)
Purpose: Used for purification of nucleic acids.
Specific Hazards:
- Phenol:chloroform:IAA is corrosive and harmful if inhaled.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
- Chemical fume hood
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
-
Liquid Waste:
- Transfer liquid waste to labeled container designated for phenol:chloroform:IAA waste.
-
Solid Waste:
- Tubes/tip/gloves with residual phenol:chloroform:IAA should be stored in chemical fume hood for no less than 24hrs and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Proteinase K
Purpose: Enzyme used to degrade proteins during DNA isolation.
Specific Hazards:
- None.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
Waste Disposal:
- No requirements.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Sodium acetate
Purpose: Used to precipitate nucleic acids.
Specific Hazards:
- Skin irritant.
Personal Protective Equipment:
- REQUIRED:
- Gloves
Waste Disposal:
-
Liquid Waste:
- Dispose in sink.
-
Solid Waste:
- Tubes/tip/gloves with residual sodium acetate can be disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Ethidium bromide (EtBr)
Purpose: Use for staining nucleic acids in agarose gels.
Specific Hazards:
- Mutagen and potential carcinogen.
Personal Protective Equipment:
-
REQUIRED:
- Gloves
-
WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
NOTE: Disposal instructions are only for dilute EtBr concentrations (< 10ug/mL) that are used in the lab. Concentrations higher than this are hazardous waste and should be placed in a labeled, dedicated container.
-
Liquid Waste:
- Dispose in sink.
-
Solid Waste:
- Tubes/tip/gloves/gels should be double bagged, labelled "non-hazardous", and then disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24
Qiagen Kits
Purpose: Use for isolation of nucleic acids.
Specific Hazards:
- Harmful, irritant.
NOTE: Do not combine kit reagents with bleach (sodium hypchlorite)!
Personal Protective Equipment:
- REQUIRED:
- Gloves
- WHEN SPLASH POTENTIAL EXISTS
- Safety goggles
- Lab Coat
Waste Disposal:
-
Liquid Waste:
- Transfer liquid waste to labeled container designated for Qiagen Kit waste.
-
Solid Waste:
- Tubes/tip/gloves with residual sodium acetate can be disposed in regular trash.
Prepared by:
- Name: Sam White
- Date: 2016-10-24